Likokoana-hloko
Saense
Mekhahlelo e nepahetseng e phahameng ea motlakase le ea matsoho e fumane lits'ebetso tse ngata lefapheng la mahlale a baeloji, e nolofalletsang bafuputsi ho etsa boemo bo nepahetseng le ho tsamaisa lisampole, lisebelisoa le lits'ebetso tsa litšoantšo.Mekhahlelo ena e fana ka ho nepahala ho ikhethang, ho pheta-pheta, le botsitso, e ba etsang lisebelisoa tsa bohlokoa bakeng sa liteko le litlhahlobo tse fapaneng.Tlhalosong ena e qaqileng, ke tla tšohla ts'ebeliso ea mekhahlelo e phahameng ea ho falla libakeng tse tharo tsa bohlokoa tsa lipatlisiso tsa baeloji: microscopy, cell manipulation, le boenjiniere ba lisele.
Microscopy:
Mekhahlelo ea phalliso e nepahetseng haholo e bapala karolo ea bohlokoa mekhoeng e tsoetseng pele ea maekkorosekopo joalo ka confocal microscopy, super-resolution microscopy, le live-cell imaging.Mekhahlelo ena e lumella bafuputsi ho beha mehlala le sepheo ka nepo, ho thusa ho nkuoa ha litšoantšo tsa boleng bo holimo tse nang le li-artifact tse nyane haholo.Ka ho kopanya methati ea phallo ea enjene ho lits'ebetso tsa microscope, bo-ramahlale ba ka iketsetsa liprothokholo tse rarahaneng tsa ho nahana, ho kenyeletsoa litšoantšo tse nang le mahlakore a mangata, ho nka nako e telele, le ho nkuoa ha Z-stack.Moetso ona o ntlafatsa katleho ea liteko le ho fokotsa liphoso tse bakoang ke basebelisi, e leng se lebisang ho liphetho tse nepahetseng le tse ka hlahisoang hape.
Tšebeliso ea Lisele:
Ho baeloji ea lisele le theknoloji ea baeloji, taolo e nepahetseng ea lisele e bohlokoa bakeng sa lits'ebetso tse fapaneng, ho kenyeletsoa tlhahlobo ea sele e le 'ngoe, ho hlopha lisele le ho kenya microinjection.Mekhahlelo e nepahetseng ea ho falla e thusa bafuputsi ho beha li-micropipettes, li-microelectrodes, lisebelisoa tsa microfluidic tse nang le ho nepahala ha sub-micrometer, ho tsamaisa mekhoa e bonolo e kang ho patch clamping, ente ea intracellular, le ho ts'oaroa ha lisele.Mekhahlelo ena e boetse e ts'ehetsa nts'etsopele ea lits'ebetso tsa automated cell manipulation, moo libetsa tsa liroboto tse nang le mekhahlelo ea ho falla li ka etsa liteko tse phahameng ka ho hlopha lisele kapa liteko tsa tlhahlobo.
Boenjineri ba Tishu:
Boenjiniere ba mahlahahlaha bo ikemiselitse ho theha lisele tse sebetsang le litho tsa 'mele ka ho kopanya lisele, biomaterials, le lintlha tsa biochemical.Mekhahlelo ea phalliso e nepahetseng haholo e thusa ho theheng meaho ea lisele tse nang le tlhophiso e nepahetseng ea sebaka le lijeometri tse rarahaneng.Bafuputsi ba ka sebelisa mekhahlelo ena ho laola ho beoa ha lisele le li-biomaterials layer-by-layer, e leng ho nolofalletsang ho theha li-scaffolds tse rarahaneng tsa lisele.Ho feta moo, methati ea phalliso e kopantsoeng le mahlale a bioprinting e lumella ho beoa ha li-bioinks ka nepo le ho fetisoa ha li-bioinks, e leng se nolofalletsang ho etsoa ha likarolo tse rarahaneng tsa lisele tse tharo.Likhatelo-pele tsena tsa boenjiniere ba lisele li na le ts'episo e kholo bakeng sa meriana e nchafatsang le ho sibolloa ha lithethefatsi.
Ka bokhuts'oane, methati e nepahetseng e phahameng ea motlakase le ea matsoho e fetotse lefapha la mahlale a baeloji ka ho fana ka bokhoni bo nepahetseng le bo tšepahalang ba ho beha maemo.Lisebelisoa tsa bona ho microscopy, manipulation cell, le boenjineri ba lisele li entse lipatlisiso tse tsoetseng pele haholo libakeng tsena, tse lebisang katlehong ea ho utloisisa lits'ebetso tsa cellular, ho nts'etsapele liphekolo tse ncha, le ho theha lisele tse sebetsang.Ha thekenoloji e ntse e tsoela pele ho fetoha, re ka lebella ho kopanngoa ho eketsehileng ha mekhahlelo e phahameng ea ho falla le mekhoa e meng ea morao-rao, ho khanna mekhoa e mecha le ho sibolloa lefapheng la saense ea baeloji.